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KMID : 0545120150250081257
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 8 p.1257 ~ p.1264
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Isolation, Purification, and Structural Identification of an Antifungal Compound from a Trichoderma Strain
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Chong-Wei Li
Rui-Qing Song
Li-Bin Yang
Xun Deng
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Abstract
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Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5- cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).
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KEYWORD
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Trichoderma, biocontrol, bioactive component, antifungal, structural identification, Cytospora chrysosperma
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